How to Extract DNA From Human Cheek Cells
From your Biology Guide
Lab method for extracting DNA from human cheek cells.
- Gather together sodium chloride, liquid soap, 2 glass beakers, bottled and distilled water, 2 test tubes, test tube stoppers, glass rod, a plastic cup and rubbing alcohol.
- Place 8 grams of sodium chloride in one of the beakers and dissolve with 92 milliliters of distilled water.
- In a second beaker, combine 25 milliliters of liquid soap with 75 milliliters of distilled water.
- Pour 1 milliliter of the sodium chloride solution into a large test tube.
- Pour 10 milliliters of the bottled water into a plastic cup and swirl the water in your mouth for 30 seconds.
- Spit the water back into the cup and pour the contents into the test tube containing the sodium chloride solution.
- Add 1 milliliter of liquid soap to the test tube.
- Cover the top of the test tube with a stopper and gently mix the contents by turning the test tube upside down and right side up.
- Add 5 milliliters of the rubbing alcohol to the test tube making sure to pour it at an angle down the side of the test tube.
- Wait for about 5 minutes and watch as the DNA floats to the surface.
- Pour 1 milliliter of alcohol into the second test tube.
- Use the glass rod to remove the DNA from the first test tube by twirling the rod in a circular motion.
- Place the DNA into the second test tube containing the alcohol.
- If more DNA is desired, repeat the procedure making sure to wash your mouth more vigorously in step 5.
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